Department of Biochemistry and Molecular Biology (Division
of Cellular Siganling)
Faculty of Medicine, The University of Tokyo
We are performing research on projects as follows.
1.Metabolism and functions of lipid mediators.
Besides roles of energy supply and composition of cellular
membrane, hormone-like lipid mediators are produced and work as bioregulators
in concert with neurotransmitters or hormones.In 1991, we cloned the receptor
for platelet-activating factor (PAF) as the first successful example of lipid
mediator receptors.Following this breakthrough, we isolated several key molecules
in the biosynthesis, metabolism and cellular functions of various lipid mediators,
which include prostaglandins, leukotrienes, PAF, and lysophosphatidic acid.Mice
either lacking or overexpressing these gene have been established, and the
roles of mediators are now clarified in vivo.We found that these lipid mediators
are playing critical roles in the self-defense systems as well as neuronal
transmission and plasticity.In collaboration with lipid biochemistry techniques
of Department of Metabolome, we will find out novel lipid mediators using
many orphan GPCR and nuclear receptors.
2.Mechanism of neuronal network formation using semaphorine-deficient
We have generated Sema3A-deficient mice by homologous recombination,
and found various abnormalities in axon guidance and formation of neuronal
circuit.We are now studying the roles of Sema3A in various other systems,
identification of novel semaphoring members, and their cellular functions.Especially,
we are interested in the roles of lipid signaling molecules and lipoxygenase
reaction on the downstream action of Sema-neuropilin-plexin axis.
3.Deorphaning of lipid GPCR and dynamic changes in
lipid composition by cell stimuli
Department of Metabolome is focusing three projects: (1) deorphaning
projects of GPCR using natural lipid library, (2) analyses by mass spectrometry
dynamic changes of membrane composition upon cell stimuli (lipid metabolomics),
and (3) establishment of database and searching engine of lipid mediators.The
Department was established in2003 by donation of Shimadzu Co., Ltd, and Ono
Pharmaceutical Co., Ltd.
Expression of the biosynthetic enzyme of lipid mediators after kainite
Professor：Takao Shimizu, M.D., Ph. D.
Associate Professor：Takehiko Yokomizo, M.D., Ph.D.
Research Associate：Satoshi Ishii, Ph.D. Masahiko Taniguchi, Ph.D. Naonori
Uozumi, M.D., Ph.D.
Graduate students： doctor course 12, master course 2
Department of Metabolome
Professor：Ryo Taguchi, Ph.D.
Research Associate：Toshiaki Okuno, Ph.D.
Division of Laboratory Instrument
Research Associate：Toshie Takahashi
Home page http://biochem2.umin.jp/index.html